Month: September 2025

How pH Influences Protein Separation in Ion Exchange Chromatography

September 29, 2025

Matthew Crawford Ion exchange chromatography (IEX) is one of the most widely used techniques for protein analysis and purification. It exploits differences in the net surface charge of proteins, allowing separation based on subtle charge heterogeneities. Among the many factors…

How pH Affects Separation of Oligonucleotides in Ion Exchange Chromatography

September 16, 2025

Matthew Crawford Purifying oligonucleotides for research, diagnostics, or therapeutic applications often relies on ion exchange chromatography (IEX) due to its highly biocompatible properties during separation and good resolution. Among the various chromatographic methods available, IEX is particularly powerful because it…