Protein Purification
At Rilas we specialize in overcoming the toughest challenges in protein purification by leveraging our extensive fleet of FPLCs, HPLCs, LCMS and sample handling systems. From stability and solubility issues to achieving high purity and yield, our team uses advanced chromatography techniques—including ion exchange, size exclusion, and hydrophobic interaction—to tailor purification workflows to each protein’s unique properties. We partner with industry leading resin manufacturers to deliver the broadest necessary range of resins for each separation, as well as high-throughput instrumentation so we can rapidly develop, optimize, and scale purification protocols. Integrated LCMS analytics ensure precise monitoring at every step, enabling fast troubleshooting and confident decision-making. Whether you're working with novel constructs or scaling up production, we deliver consistent, high-quality results with speed and precision.
Ion Exchange Chromatography (IEX, AEX, CEX)
• Proteins exhibit charge character that can be utilized to separate species by relative charge under specific conditions.
• Strong and weak cation and anion exchangers are used to find the best possible separation for each construct while maintaining scalability of the separation.
• pH mapping is always performed, within tolerable pH ranges to ensure stability of constructs while determining optimal conditions for separation of closely related species.
Size Exclusion Chromatography (SEC)
• Commonly used technique in tandem with other, selective methods
• Separates based on relative size of molecules in the liquid phase
• Scalable SEC resins allow for movement from discovery to small-scale animal studies at rapid rates
• Biocompatible method for a broad range of buffer modifiers
Hydrophobic Interaction Chromatography (HIC)
• Leverages differences in hydrophobicity on molecules to separate
• Often used to separate proteins labelled with hydrophobic drug molecules (see ADC page for more!)
Affinity Purification (His-tag, Flag-tag, HA-tag, Custom)
• Sequence tagging is a common modification used to improve selectivity in early-stage protein purification
• Highly selective method removes majority of contaminants in first step
• Compatible with other methods as a capture step prior to polishing
• Highly scalable; Rilas also supports construct redesign to remove tag and affinity capture step when moving to more native sequences
Why Choose Rilas for Protein Purification?
At Rilas, we know each protein is unique and requires a specific set of conditions to achieve optimal purity. We also recognize the need for speed and reliability, and work extensively with our customers to ensure quality while moving with the speed required by early to mid-stage biotechs.
Instrumentation:
Agilent 6545XT AdvanceBio LC/Q-TOF
Agilent 1290 Infinity II HPLC/MS
Agilent 1260 HPLC/UV/ELSD