Experimental Polar Surface Area
Experimental Polar Surface Area (EPSA) is an SFC based measurement estimating the compounds’ polar surface area in a non-polar environment.
Turnaround Time:
Our high-throughput EPSA measurements can analyze >1000 compounds per week.
Benefits of EPSA Measurements
EPSA measurements can identify the molecules that are most likely to form intramolecular hydrogen bonds by comparing the calculated polar surface area to the EPSA results. If the EPSA results are lower than the calculated polar surface area the molecule will likely form internal hydrogen bonds. This is an important drug design consideration given the correlation of intramolecular hydrogen bonding with increased membrane permeability.
Instrumentation:
Agilent 1260 Infinity II SFC/MS