DNA Encoded Library Purifications
DNA Encoded Libraries are vast collections of synthetic organic
molecules linked to DNA barcodes and generated by combinatorial
chemistry technique called spit/pool synthesis. It is imperative to
remove unreacted building blocks, side products and excess reagents before subsequent biological screening. At Rilas, we are employing our high-throughput HPLC purification process at two different stages.
After the First Building Block Coupled to DNA Tag
At this stage each well contains a single target plus unreacted DNA and building block as well as excess reagents. Employing our highthroughput HPLC purification process we can purify >300 compounds per week providing >95% pure targets in each well and they are ready for the split/pool process.
At the End of the Split/Pool Process
Combining our HPLC purification expertise with our efficient mass spec deconvolution process we can design efficient purification of a mixture containing tens of thousands of samples to remove all the unwanted side products, unreacted building blocks, excess reagents and make the final library ready for biological interrogation.
Instrumentation:
Agilent 1290 Infinity II Mass Directed Automated Purification System