A World-Class Chemistry Laboratory Committed to Your Needs

Rilas Lab™

Chiral SFC Separation

  • Preparative chiral separation from milligrams up to 50 g quantities of discovery research compounds, templates, intermediates, drug candidates

  • Enantiomeric and diastereomeric excess confirmation of starting materials, intermediates and final products

  • Automated method development for chiral separations

Instrumentation

  • Agilent 1260 Infinity II SFC/MS

  • Waters SFC 80

  • BioTools Chiral R-2x

  • Buchi Rotavapors

  • Biotage V-10

HPLC/MS Purification

  • Achiral purification and reformatting mg to gram quantities of singletons and libraries of compounds

  • Automated method development for achiral separations

  • High-throughput HPLC/MS QC of compound collections up to 1000 compounds/week

  • Repeat literature analysis

  • Purity analysis by HPLC/MS

  • Sample Identification using LCMS

  • Reformatting in customer’s preferred format in up to four of the customer’s own barcoded vials


Instrumentation

  • Agilent 1290 Infinity II HPLC/MS

  • Agilent 1290 Infinity II Mass Directed Automated Purification System

  • TECAN Freedom Evo 150 Liquid Handler

  • Genevac EZ-2

Physico-Chemical Measurements

  • EPSA: SFC based measurement estimating the compounds’ polar surface area and its tendency of forming internal hydrogen bonds

  • Solubility: Measure peak area ratios relative to the peak in DMSO or methanol solution

  • Stability: Samples in aqueous solutions reanalyzed after 24 hours and compare to the original purity

  • LogD: Calculated based on retention time using a calibration curve generated by a shallow water/acetonitrile gradient

  • Glutathione Reactivity Measurement

  • Solubility and stability in simulated gastric solutions

  • Rilas can customize the measurements for any biologically relevant buffers customers require

Instrumentation

  • Agilent 1260 HPLC/UV/ELSD

  • TECAN Freedom Evo 150 Liquid Handler